Development of a CRISPR/Cas9 system for high efficiency multiplexed gene deletion in Rhodosporidium toruloides
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چکیده
منابع مشابه
Purification and characterization of a cephalosporin esterase from Rhodosporidium toruloides.
A novel cephalosporin esterase (EC 3.1.1.41) from Rhodosporidium toruloides was purified to gel electrophoretic homogeneity. The enzyme is a glycoprotein with a molecular mass of 80 kDa. Upon deglycosylation, several forms of the enzyme were observed with a molecular mass range between 60 and 66 kDa. The isoelectric point of the enzyme is approximately 5.6, with the pH optimum for activity occu...
متن کاملThe Production, Regulation and Extraction of Carotenoids from Rhodosporidium toruloides
Carotenoids are valuable pigments of commercial interest for various health benefits. There is rising demand for natural carotenoids from microorganisms, although the majority of industrially produced carotenoids are currently chemically synthesized. Rhodosporidium toruloides is oleaginous red yeast, which can produce large amounts of carotenoids and lipids simultaneously. It is also able to as...
متن کاملHigh-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture
Microbial lipid production by the oleaginous yeast Rhorosporidium toruloides Y4 was studied using glucose as carbon source, in order to realize igh-density cell culture. Batch cultures demonstrated that there was little inhibitory effect with a substrate concentration of up to 150 g l−1. Flask ed-batch cultures were run for 25 days and reached a dry biomass and cellular lipid content of 151.5 g...
متن کاملDevelopment of a multiplexed microbioreactor system for high-throughput bioprocessing.
A multiplexed microbioreactor system for parallel operation of multiple microbial fermentation is described. The system includes miniature motors for magnetic stirring of the microbioreactors and optics to monitor the fermentation parameters optical density (OD), dissolved oxygen (DO), and pH, in-situ and in real time. The microbioreactors are fabricated out of poly(methylmethacrylate)(PMMA) an...
متن کاملD-amino-acid oxidase gene from Rhodotorula gracilis (Rhodosporidium toruloides) ATCC 26217.
The complete nucleotide sequence of the DAO1 gene encoding D-amino-acid oxidase (DAAO) in the yeast Rhodotorula gracilis (Rhodosporidium toruloides) ATCC 26217 has been determined. The primary structure of DAAO was deduced from the nucleotide sequence of a cDNA clone that covered the entire amino acid coding sequence. Comparison of cDNA and genomic sequences of DAO1 revealed the presence of fiv...
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 2019
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.27001